Biocontrol Using Pythium oligandrum during Malting of Fusarium-Contaminated Barley

This study investigates the potential of Pythium oligandrum (strains M1 and 00X48) as a biocontrol agent in suppressing growth Fusarium culmorum production mycotoxins during malting naturally contaminated barley (Hordeum vulgare). The effects on F. culmorum-infected malt (BM) were evaluated through real-time PCR its impact mycotoxin was determined by quantitative analysis deoxynivalenol (DON) deoxynivalenol-3-glucoside (D3G). effect treatment BM beer quality also European Brewery Convention (EBC) standard methods. Optimal with P. strains 00X48 yielded 59% 48% reduction contamination, 37% 17% lower DON, 27% 32% D3G, respectively. treated both exhibited enhancement; produced from strain resulted no deterioration 26% 18% less DON respectively, transferred to final product.